Solutions, methods, and processes for deprotection of polynucleotides

US8552174B2 · kind B2 · utility

Assignees

• AGILENT TECHNOLOGIES, INC. · US
• The Regents of the University of Colorado · US

Inventors

• Douglas J. Dellinger · Boulder, US
• Zoltan Timar · Boulder, US
• Agnieszka Sierzchala · Boulder, US
• Geraldine F. Dellinger · Boulder, US
• Marvin H. Caruthers · Boulder, US
• Joel Myerson · Berkeley, US

Key dates

• Filing date: Mar 23, 2006
• Grant date: Oct 8, 2013
• Expiry date: Apr 2, 2030

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/55
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Methods of deprotecting polynucleotides are disclosed. One aspect of the method of deprotecting polynucleotides, among others, includes: providing a polynucleotide, wherein the polynucleotide includes at least one nucleotide monomer that has at least one protecting group selected from the following: a base having a protecting group, a 2′-hydroxyl protecting group, and a combination thereof, and deprotecting at least one of the protecting groups of the polynucleotide by introducing the polynucleotide to a solution including an α-effect nucleophile.