Phosphotriesterase from agrobacterium radiobacter P230

US8557547B2 · kind B2 · utility

Assignee

• COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION · AU

Inventors

• Irene Horne · Gungahlin, AU
• Tara D. Sutherland · North Canberra, AU
• Rebecca Harcourt · Sydney, AU
• Robyn Russell · Tuggeranong, AU
• John Oakeshott · Tuggeranong, AU

Key dates

• Filing date: Oct 12, 2012
• Grant date: Oct 15, 2013
• Expiry date: Oct 12, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention provides a phosphotriesterase enzyme identified from an Agrobacterium radiobacter strain isolated from soil that hydrolyses OP pesticides, and the gene encoding that enzyme. The invention also provides mutants of the identified phosphotriesterase enzyme which have altered substrate specificity. The use of these enzymes in bioremediation strategies is also provided.