Nuclease reduction
US8557553B2 · kind B2 · utility
1Cited by
3References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 19, 2008 |
| Grant date | Oct 15, 2013 |
| Priority date | — |
| Expiry date | Oct 11, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/96
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to a method for reducing nuclease activity in a subtilisin solution obtained from a fermentation broth including: a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5; b) adding a polyol; and c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C. In accordance with the present disclosure, the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 60% of the initial value.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.