Fluorescent finger prints for indirect detection in isotachophoresis
US8562804B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 31, 2011 |
| Grant date | Oct 22, 2013 |
| Priority date | — |
| Expiry date | Dec 7, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Indirect detection and/or identification of analytes by ITP can be enhanced by adding a mixture of labeled carrier ampholytes (CAs) to the sample to provide a continuous range of mobility markers. Each analyte can be detected and quantified by corresponding gaps in the CA signal. This approach does not require a priori choice of fluorophores and can be readily applied (without extensive and specific design) to a wide range of analytes. Analyte identification can be expedited by computing a normalized signal integral (NSI) from the CA signals. Empirical calibrations can relate the NSI to effective mobility. Effective mobility results under two or more different pH conditions can be used to determine analyte pKa and fully ionized mobility, which are analyte properties that can facilitate analyte identification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.