Patent · US Active

Method for increasing recloning efficiency

US8563303B2 · kind B2 · utility

0Cited by

5References

17Claims

0Family size

Assignee

BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG · DE

Inventors

Juergen Fieder · Unterstadion, DE
Lore Florin · Biberach, DE
Hitto Kaufmann · Ulm, DE
Thomas Krieg · Erbach, DE
Melanie Briegel · Riedlingen, DE

Key dates

Filing date	Mar 16, 2009
Grant date	Oct 22, 2013
Priority date	—
Expiry date	Apr 8, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2510/02
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to the field of cell culture technology and relates to methods of replicating/cloning cells, preferably cell lines which are important for the production of biopharmaceuticals. The invention also relates to methods of preparing proteins using cells that have been obtained and replicated by single cell deposition and compositions which make it possible to replicate individual cells.By using IGF particularly in conjunction with HSA in the culture medium after recloning, the recloning efficiency and hence the quantity of clones obtained can be increased significantly.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.