Methods of culturing cells in a medium comprising transforming growth factor beta 1 and basic fibroblast growth factor
US8563311B2 · kind B2 · utility
17Cited by
5References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 8, 2012 |
| Grant date | Oct 22, 2013 |
| Priority date | — |
| Expiry date | May 8, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.