Methods of nucleic acid synthesis using particular crowding agents and concentrations
US8568982B2 · kind B2 · utility
1Cited by
8References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 10, 2007 |
| Grant date | Oct 29, 2013 |
| Priority date | — |
| Expiry date | Apr 5, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method of nucleic acid synthesis and/or amplification, and/or of improving the efficiency, activity and/or stability of at least one nucleic acid-modifying enzyme, comprising carrying out the method in the presence of (a) at least one organic-based macromolecule having a molecular weight of 50 kDa to 500 kDa and neutral surface charge; or (b) at least one organic-based macromolecule of radius 2 to 50 nm and neutral surface charge. There is also provided a method of determining the optimum crowding conditions of macromolecule(s) in solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.