Methods and compositions for detecting rare cells from a biological sample

US8569009B2 · kind B2 · utility

Assignee

• AVIVA BIOSCIENCES CORPORATION · US

Inventors

• Ping Lin · San Diego, US
• Andrea Ghetti · Vimercate, IT
• Wenge Shi · San Marcos, US
• Mengjia Tang · San Diego, US
• Gioulnar I. Harvie · San Diego, US
• Huimin Tao · San Diego, US
• Guoliang Tao · San Diego, US
• Lei Wu · San Diego, US
• David E. Cerny · Lilburn, US
• Jia Xu · San Diego, US

Key dates

• Filing date: Aug 20, 2007
• Grant date: Oct 29, 2013
• Expiry date: Aug 19, 2029

Classification

• Technology area (CPC —): General

Abstract

Provided are methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells, particularly including debulking that uses a microfabricated filter for filtering samples. The enriched rare cells can be used in a downstream process such as identification, characterization or growth in culture, or in other ways. Also included is a method of determining tumor aggressiveness or the number or proportion of cancer cells in the enriched sample by detecting telomerase activity, nucleic acid or expression after enrichment of rare cells. Also provided is an efficient, rapid method to specifically remove red and white blood cells from a biological sample containing at least one of the cell types, leading to enrichment of rare target cells including circulating tumor (CTC), stromal, mesenchymal, endothelial, fetal, stem, or non-hematopoietic cells et cetera from a blood sample.