Method of producing a population of cells

US8569060B2 · kind B2 · utility

Assignee

• The University Of Queensland · AU

Inventors

• Nicholas Timmins · Scarborough, CA
• Lars K. Nielsen · Brisbane, AU
• Emma Louise Palfreyman · Brisbane, AU

Key dates

• Filing date: Jan 8, 2009
• Grant date: Oct 29, 2013
• Expiry date: Jan 8, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2501/39
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

An in vitro or ex vivo method of producing a population of lineage committed haematopoietic progenitor or mature haematopoietic cells other than cells of the neutrophil lineage, including the steps of providing a population of haematopoietic progenitor cells; and culturing the haematopoietic progenitor cells in an animal cell culture medium including one or more cytokines that differentiate the haematopoietic progenitor cells into lineage committed haematopoietic progenitor and/or mature haematopoietic cells, under static conditions until the cells are at a cell density at which oxygen transfer via the surface of the culture medium is insufficient for growth of the progenitor cells and progeny thereof under static conditions, and then agitating the culture medium to produce a population of lineage committed haematopoietic progenitor or mature haematopoietic cells other than cells of the neutrophil lineage.