System and method for high throughput cell analysis and sorting
US8569069B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 19, 2011 |
| Grant date | Oct 29, 2013 |
| Priority date | — |
| Expiry date | Oct 11, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M47/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A sorting methodology in which bulk analysis of samples that have a low probability of containing a rare particle is performed first. Only those samples in which the bulk analysis has detected one or more rare particles are subjected to a flow cytometry process to isolate the rare particle(s). In one embodiment, a microwell plate provides for high throughput sorting in an enclosed environment. The microwell plate includes a plurality of wells and a microfluidic layer adapted for flow cytometry wherein particles from a given well are interrogated and sorted into other wells. The microfluidic layer advantageously includes fluid switches to permit bi-directional flow cytometry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.