Methods for preparing sequencing libraries

US8574832B2 · kind B2 · utility

Assignees

• Massachusetts Institute of Technology · US
• THE GENERAL HOSPITALL CORPORATION · US

Inventors

• Mazhar Adli · Malden, US
• Bradley E. Bernstein · Cambridge, US
• Tarjei Sigurd Mikkelsen · Dublin, US

Key dates

• Filing date: Feb 3, 2010
• Grant date: Nov 5, 2013
• Expiry date: Feb 8, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1096
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Improvements in chromatin immunoprecipitation-high throughput sequencing techniques has allowed the creation of chromatin maps from limited biological sample sizes that cannot be evaluated using conventional chromatin immunoprecipitation-sequencing protocols. For example, a modified universal primer is utilized that incorporates restriction enzymes into chromatin immunoprecipitation fragments before amplification. The improved method allows the sample sizes to be several orders of magnitude less than that required for standard ChIP-Seq techniques.