Thermostable 1,5-anhydroglucitol dehydrogenase, and method for measurement of 1,5-anhydroglucitol by using the same

US8574882B2 · kind B2 · utility

Assignee

• NIPPON KAYAKU KABUSHIKI KAISHA · JP

Inventors

• Hideki Yoshioka · Takasaki, JP
• Shuhei Tsukamoto · Takasaki, JP
• Minoru Masuda · Ageo, JP
• Reiko Machida · Takasaki, JP
• Yoshihiko Umegae · Takasaki, JP
• Masahiko Yabuuchi · Saitama, JP

Key dates

• Filing date: Jun 18, 2009
• Grant date: Nov 5, 2013
• Expiry date: Nov 21, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/32
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A protein comprising an amino acid sequence having at least one mutation selected from a Gly-4 to Ala mutation, a Glu-6 to His mutation, a Ser-14 to Thr mutation, an Ala-37 to Thr or Arg mutation, a Pro-50 to Gln mutation, a Glu-67 to Gly mutation, an Asp-80 to Tyr mutation, a Val-93 to Met mutation, an Arg-156 to Pro mutation, a Leu-164 to Met mutation, an Asn-202 to Asp mutation, a Thr-235 to Ala mutation, an Asn-348 to Tyr mutation, a Gly-362 to Arg mutation and a Val-473 to Ala mutation in the amino acid sequence depicted in SEQ II NO:4. (2) A thermostable protein which comprises an amino acid sequence derived from the amino acid sequence having at least one variation described in (1) and having 1,5-anhydroglucitol dehydrogenase activity. These proteins act specifically on 1,5-anhydroglucitol (1,5-AG), have thermal stability and exhibit excellent storage stability.