Production of functionalized linear DNA cassette and quantum dot/nanoparticle mediated delivery in plants

US8575424B2 · kind B2 · utility

Assignee

• DOW AGROSCIENCES LLC · US

Inventors

• Kerrm Y. Yau · Carmel, US
• Jayakumar P. Samuel · Carmel, US
• Frank Burroughs · Noblesville, US
• Narasimha C. Samboju · Carmel, US
• Steven R. Webb · Westfield, US

Key dates

• Filing date: Jul 7, 2011
• Grant date: Nov 5, 2013
• Expiry date: Nov 15, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/13
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall include use of nanoparticles. In some embodiments, the cell comprising a cell wall is a cultured plant cell. Methods include genetically or otherwise modifying plant cells and for treating or preventing disease in any plant, especially crop plants. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with functionalized linear nucleic acid cassette molecules.