Methods and compositions for amplification and detection of MicroRNAs
US8580494B2 · kind B2 · utility
1Cited by
4References
19Claims
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Key dates
| Filing date | Aug 24, 2007 |
| Grant date | Nov 12, 2013 |
| Priority date | — |
| Expiry date | Dec 11, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and system for amplifying non-coding RNA, microRNA, and small polynucleotide sequences through the generation of a pool of signature sequences to the target sequences. The target sequences can be amplified through DNA synthesis, RNA synthesis, or the combination of DNA and RNA synthesis. The amplification of signature sequences provides an efficient and reproducible mechanism to determine the presence or absence of the miRNAs, to analyze the quantities of the target miRNAs, and for miRNA profiling. The method may also be used for screening for unknown non-coding RNAs, including novel miRNAs.
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