Methods and compositions for use in analyte detection using proximity probes
US8580504B2 · kind B2 · utility
6Cited by
5References
26Claims
0Family size
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Key dates
| Filing date | Jan 24, 2011 |
| Grant date | Nov 12, 2013 |
| Priority date | — |
| Expiry date | Jan 24, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/542
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compositions for detecting an analyte in a sample are provided. In practicing the subject methods, a sample is combined with at least a pair of proximity probes that each include an analyte binding domain and a nucleic acid domain. The resultant mixture is then contacted with a pair of asymmetric nucleic acid connectors. Proximity dependent connector mediated interaction between the nucleic acid domains of the proximity probes is then detected to determine the presence of the analyte in the sample. Also provided are kits and systems for practicing the subject methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.