Plant peptide gamma-zein for delivery of biomolecules into plant cells

US8581036B2 · kind B2 · utility

Assignee

• DOW AGROSCIENCES LLC · US

Inventors

• Narasimha C. Samboju · Carmel, US
• Jayakumar P. Samuel · Carmel, US
• Gaofeng Lin · Beijing, CN
• Steven R. Webb · Westfield, US
• Frank Burroughs · Noblesville, US

Key dates

• Filing date: Mar 8, 2011
• Grant date: Nov 12, 2013
• Expiry date: Dec 23, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8206
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.