Single photon spectrometer

US8582098B2 · kind B2 · utility

Assignee

• The Research Foundation of the State University of New York · US

Inventors

• Andriy Tsupryk · Coram, US
• Ivan Tovkach · Centereach, US
• Dmitriy Gavrilov · Setauket, US
• Georgiy Gudkov · Coram, US
• Vera Gorfinkel · Stony Brook, US
• Boris Gorbovitski · Stony Brook, US
• Dmytro Gudkov · Stony Brook, US

Key dates

• Filing date: Oct 24, 2008
• Grant date: Nov 12, 2013
• Expiry date: Feb 9, 2030

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A fiberized single photon sensitive spectrometer based on a 32-channel PMT sensor is highly sensitive with broad detection dynamic range. The spectrometer enables accurate and high speed detection, identification and analysis of biological samples labeled with multiple fluorescent markers, such as compositions of multi-color fluorescence signals or radiation emitted by multiple fluorescence dyes. A fiberized optical input of the spectrometer allows an easy and efficient coupling to any measurement system based on fiber collection of the analyzed fluorescence. The spectrometer provides highly accurate DNA sequencing. A 32 channel PMT single photon detector has a detection dynamic range of more than 20 bits and has a frame rate of about 3300 frames per second. The dynamic range of the detector's pixels reaches 108 photocounts per second.