Patent · US Active

Nucleic acid amplification in the presence of modified randomers

US8592184B2 · kind B2 · utility

0Cited by

19References

9Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Dieter Heindl · Munich, DE
Waltraud Ankenbauer · Penzberg, DE
Frank Laue · Kempten (Allgäu), DE

Key dates

Filing date	Dec 12, 2012
Grant date	Nov 26, 2013
Priority date	—
Expiry date	Dec 12, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/686
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.