Small RNA-dependent translational regulatory system in cell or artificial cell model
US8592569B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2008 |
| Grant date | Nov 26, 2013 |
| Priority date | — |
| Expiry date | Feb 21, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.