Method and apparatus for conducting an assay
US8597882B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 3, 2012 |
| Grant date | Dec 3, 2013 |
| Priority date | — |
| Expiry date | Feb 3, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/0444
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to methods and apparatus for conducting nucleic acid sequencing by pyrosequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support surface within each well, wherein the support surface is adapted to immobilize a first binding partner or selectively immobilize a second binding partner. The method further comprises the steps of binding or immobilizing the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.