Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US8597891B2 · kind B2 · utility
108Cited by
100References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 6, 2012 |
| Grant date | Dec 3, 2013 |
| Priority date | — |
| Expiry date | Jul 6, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.