Use of HSA-producing cells

US8597909B2 · kind B2 · utility

Assignee

• BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG · DE

Inventors

• Lore Florin · Biberach, DE
• Rebecca Bischoff · Warthausen, DE
• Juergen Fieder · Unterstadion, DE
• Hitto Kaufmann · Ulm, DE
• Thomas Krieg · Erbach, DE

Key dates

• Filing date: Sep 9, 2009
• Grant date: Dec 3, 2013
• Expiry date: Nov 17, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2510/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types.