Bioreactor for quantification of headspace VOC content from cultures

US8597941B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Hye Won Shin · Seoul, KR
• Brandon J. Umber · Newport Beach, US
• Donald R. Blake · Wichita, US
• Dan M. Cooper · Irvine, US

Key dates

• Filing date: Sep 3, 2010
• Grant date: Dec 3, 2013
• Expiry date: Jun 13, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M41/34
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A bioreactor for sampling the headspace above cells includes a glass reactor vessel having an interior space and a plurality of valves operatively coupled to the interior space of the glass reactor vessel. The bioreactor includes a sample holder configured to reside in the interior space. The interior space includes a charge of air with a known composition of volatile organic gases and, in some instances, doped with carbon dioxide. The sample holder may hold cells, bacteria, or viruses. The headspace may be sampled after incubation to determine the VOC content or content of specific VOC constituents. Examples include acetaldehyde and hexanaldehyde.