Tol1 factor transposase and DNA introduction system using the same
US8598328B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 6, 2007 |
| Grant date | Dec 3, 2013 |
| Priority date | — |
| Expiry date | Dec 15, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An object is to provide a Tol1 element transposase and a use thereof. Provided is a Tol1 element transposase containing (a) a protein having the amino acid sequence of SEQ ID No: 1 or (b) a protein having an amino acid sequence homologous to the amino acid sequence of SEQ ID NO: 1 and having an enzymatic activity for transferring Tol1 element. Further, provided are a polynucleotide encoding the transposase and an expression construct containing the polynucleotide therein. The present invention also provides a DNA introduction system including (a) a donor factor having such a structure that a desired DNA is inserted in a transposase gene-defected Tol1 element and (b) a helper factor containing the transposase or the polynucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.