Compositions and methods for using a solid support to purify DNA

US8598338B2 · kind B2 · utility

Assignee

• QIAGEN North American Holdings, Inc · US

Inventors

• Robert Jackson Bair · Plymouth, US
• Kristen Campbell Benedict · Maple Grove, US
• Wendy J. Kivens · Edina, US
• Robert Kwiatkowski · Verona, US
• Kim Paulsen · Malung, SE
• Daniel A. Strom · Minneapolis, US
• John Wages · Pacifica, US

Key dates

• Filing date: Mar 5, 2010
• Grant date: Dec 3, 2013
• Expiry date: Mar 7, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1006
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides methods for isolating substantially pure and undegraded DNA from biological material via a solid support. The methods may use a DNA lysing solution that comprises a surfactant, a DNA-complexing salt (e.g., a lithium salt), and a buffer. Alternatively, the methods may use a solid support pre-treated with the DNA lysing solution.