GLP-1 receptor agonist bioassay
US8603761B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 30, 2012 |
| Grant date | Dec 10, 2013 |
| Priority date | — |
| Expiry date | Nov 30, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/705
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein are cell-based bioassays for measuring GLP-I receptor agonist activity of test compounds, such as GLP-I receptor agonist compounds. Exemplary GLP-I receptor agonist compounds include exendins, exendin analogs, GLP-1(7-37), and GLP-1(7-37) analogs. The bioassays are useful for quantitatively determining cAMP generated in samples containing GLP-I receptor agonist compounds (e.g., exenatide) and 6-23 (clone 6) cells having GLP-I receptors, whereby the amount of cAMP generated can be correlated to the GLP-I receptor agonist activity of the GLPI receptor agonist compounds (e.g., exenatide). Suitable cell-based bioassays include enzyme-linked immunosorbent assays and homogeneous time-resolved fluorescence assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.