Patent · US Active

Method for inserting a nucleic acid of interest into a prokaryotic or eukaryotic cell by homologous recombination

US8609419B2 · kind B2 · utility

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24Claims

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Assignee

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR

Inventors

Robert Fuchs · Cincinnati, US
Marc Bichara · Vendenheim, FR

Key dates

Filing date	Oct 27, 2003
Grant date	Dec 17, 2013
Priority date	—
Expiry date	Feb 26, 2027

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/902
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention concerns the use of a mutagenic agent blocking DNA replication in the cell for inserting in vitro a nucleic acid of interest inside a predetermined nucleotide sequence present in a chromosome contained in a prokaryotic or eukaryotic cell, said nucleic acid of interest being, prior to its insertion, included in a DNA vector which replicates in said prokaryotic or eukaryotic host cell.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.