Polymerase incorporation of non-standard nucleotides
US8614072B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jun 16, 2009 |
| Grant date | Dec 24, 2013 |
| Priority date | — |
| Expiry date | Aug 9, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2525/117
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosed invention teaches processes to amplify oligonucleotides by contacting templates and primers with DNA polymerases and triphosphates of non-standard nucleotides, which form nucleobase pairs fitting the standard Watson-Crick geometry, but joined by hydrogen bonding patterns different from those that join standard A:T and G:C pairs. Thus, this invention relates to nucleotide analogs and their derivatives that, when incorporated into DNA and RNA, expand the number of replicatable nucleotides beyond the four found in standard DNA and RNA. The invention further relates to polymerases that incorporate those non-standard nucleotide analogs into oligonucleotide products using the corresponding triphosphate derivatives, and more specifically, polymerases and non-standard nucleoside triphosphates that support the polymerase chain reaction (PCR), including PCR where the products contain more than one non-standard nucleotide unit.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.