Method for preparing nucleotides and related analogues by synthesis on soluble substrate, and biological tools thus prepared
US8614312B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 23, 2009 |
| Grant date | Dec 24, 2013 |
| Priority date | — |
| Expiry date | Nov 4, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H19/16
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention concerns a method for preparing monomer nucleotides or nucleotide analogues comprising the steps of: (1) coupling a soluble polyethylene glycol support provided with at least one diacid or ether-acid linker and a monomer nucleoside or nucleoside analogue to an amine group or hydroxyl group of the nucleoside with the aid of a coupling agent; (2) at least one step for phosphorylation of said nucleoside or nucleoside analogue coupled to said support with a phosphorylation agent; (3) cleavage of said support and recovery of at least one monomer nucleotide or nucleotide analogue. The nucleotides prepared are biological tools.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.