Nucleic acid binding proteins
US8617807B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 14, 2006 |
| Grant date | Dec 31, 2013 |
| Priority date | — |
| Expiry date | Oct 11, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1037
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method for designing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an alpha-helical zinc finger nucleic acid binding motif in the protein is determined as follows: if base 4 in the quadruplet is A, then position +6 in the alpha-helix is Glu, Asn or Val; if base 4 in the quadruplet is C, then position +6 in the alpha-helix is Ser, Thr, Val, Ala, Glu or Asn; if base 4 of the quadruplet is G, the position +6 in the alpha helix is Arg or Lys; if position 4 in the quadruplet is T, then position +6 in the alpha helix is Ser, Thr, Val or Lys.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.