Cultispot assay

US8617808B2 · kind B2 · utility

Assignee

• MABTECH AB · SE

Inventors

• Sten Braesch-Andersen · Nacka, SE
• Staffan Paulie · Nacka, SE

Key dates

• Filing date: Apr 29, 2005
• Grant date: Dec 31, 2013
• Expiry date: Apr 23, 2027

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides a method for detecting the production of one or more analytes by cells in a sample. The method uses one or more immobilized binding proteins to capture one or more analytes released from cells. The, or each, binding protein is localized at one or more discrete locations on a solid surface within a well. Cells are introduced into the well and cultured under conditions suitable for release of the analyte(s) and for binding of the analyte(s) to the binding protein(s). Restricting the binding protein(s) to one or more discrete locations within the well, rather than coating the entire surface of the well, results in increased sensitivity of the assay.