Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US8617814B2 · kind B2 · utility
44Cited by
3References
18Claims
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Key dates
| Filing date | Jul 9, 2012 |
| Grant date | Dec 31, 2013 |
| Priority date | — |
| Expiry date | Jul 9, 2032 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
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