Photoactivable fluorescent dyes for optical microscopy and imaging techniques

US8617827B2 · kind B2 · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Stefan W. Hell · Oebisfelde, DE
• Vladimir N. Belov · Oettingen, DE
• Vadim P. Boyarskiy · Saint Petersburg, RU
• Christian Wurm · Oettingen, DE
• Stefan Jakobs · Oettingen, DE
• Claudia Geisler · Bad Gandersheim, DE

Key dates

• Filing date: Sep 10, 2009
• Grant date: Dec 31, 2013
• Expiry date: Sep 10, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C09B11/24
• WIPO field: Basic materials chemistry
• WIPO sector: Chemistry

Abstract

The present invention relates to novel photoactivable rhodamine or carbopyronine derivatives of the following general formulae G1-G4 (I), G1: A1=O, A2=N, A3=C; G2: A1=S, A2=N, A3=C; G3: A1=O, A2=O, A3=N; G4: A1=S, A2=O, A3=N; comprising UV light absorbing chromophores which after photolysis by irradiation at 254-490 nm (preferably at 375-420 nm) generate fluorescent rhodamine or carbopyronine derivatives and small non-toxic fragments such as N2 or N2O. The invention also relates to methods for preparing such compounds and to the use of these compounds for optical microscopy and imaging techniques.