Patent · US Active

Recombinant peptide production using a cross-linkable solubility tag

US8617843B2 · kind B2 · utility

0Cited by

55References

12Claims

0Family size

Assignee

E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

Albert W. Alsop · Wilmington, US
Qiong Cheng · Wilmington, US
Linda Jane Solomon · Wilmington, US
Stephen R. Fahnestock · Wilmington, US
Tanja Maria Gruber · Media, US
Pierre E. Rouviere · Wilmington, US

Key dates

Filing date	Apr 6, 2011
Grant date	Dec 31, 2013
Priority date	—
Expiry date	Jul 26, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/00
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.