Polynucleotides encoding ketoreductases for producing stereoisomerically pure statins and synthetic intermediates therefor

US8617864B2 · kind B2 · utility

Assignee

• Codexis, Inc. · US

Inventors

• Lorraine Giver · Sunnyvale, US
• Lisa Marie Newman · San Jose, US
• Emily Mundorff · Garden City, US
• Gjalt W. Huisman · Redwood City, US
• Stephane J. Jenne · Foster City, US
• Jun Zhu · Chandler, US
• Behnaz Behrouzian · South San Francisco, US
• John H. Grate · Los Altos, US
• James Lalonde · Palo Alto, US

Key dates

• Filing date: Aug 9, 2012
• Grant date: Dec 31, 2013
• Expiry date: Aug 9, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P7/62
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.