Solubilization and purification of a target protein fused to a mutant maltose-binding protein
US8623615B2 · kind B2 · utility
2Cited by
1References
15Claims
0Family size
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Key dates
| Filing date | Apr 1, 2009 |
| Grant date | Jan 7, 2014 |
| Priority date | — |
| Expiry date | May 22, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions are provided that relate to a composition that includes a modified maltose-binding protein (MBP) which when fused to a protein results in an increase in binding affinity for maltodextrin compared with the wild type MBP fused to the protein, the modified MBP maintaining enhanced solubility. The modification includes a mutation selected from the group consisting of: F68L, I318V, Q326R, V344M, and T372TTTITITTTLGIEGR387 or consists of two or more mutations selected from the group consisting of: F68L, S146T, A313V, I318V, I318A, Q326R, V344M and T372TTTITITTTLGIEGR387 mutants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.