Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract
US8623626B2 · kind B2 · utility
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Key dates
| Filing date | Oct 26, 2010 |
| Grant date | Jan 7, 2014 |
| Priority date | — |
| Expiry date | Apr 11, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
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