Patent · US Active

Method of protein refolding with ion exchange resins and the application of the same

US8624005B2 · kind B2 · utility

0Cited by

0References

10Claims

0Family size

Assignee

Tianjin University · CN

Inventors

Yan Sun · Tianjin, CN
Guozhen Wang · Zhejiang, CN
Qinghong Shi · Tianjin, CN
Xiaoyan Dong · Tianjin, CN

Key dates

Filing date	Jun 30, 2011
Grant date	Jan 7, 2014
Priority date	—
Expiry date	Jun 30, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K1/18
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for protein refolding with an ion exchange resin. The method includes (a) choosing an ion exchange resin that having charged groups with the same sign as a net charge of a denatured protein to be refolded; (b) removing heterogeneous ions from the ion exchange resin by washing the ion exchange resin sequentially with saline solution and deionized water, to prepare the ion exchange resin; (c) mixing the ion exchange resin with a refolding buffer thoroughly, then adding the denatured protein to the refolding buffer and allowing the denatured protein to refold; and then (d) collecting the supernatant by centrifugation or settlement, to obtain a solution containing the refolded protein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.