Patent · US Active

Method for isolating and purifying nucleic acids

US8624020B2 · kind B2 · utility

7Cited by

4References

19Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Ralf Himmelreich · Langenfeld, DE
Sabine Werner · Mülheim an der Ruhr, DE

Key dates

Filing date	Sep 2, 2009
Grant date	Jan 7, 2014
Priority date	—
Expiry date	Nov 15, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/1006
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials, using a wash buffer comprising an alcohol having 1 to 3 carbon atoms and at least one further solvent selected from the group consisting of alkane diols and alkane triols having 2 to 6 carbon atoms, monocarboxylic acid esters and dicarboxylic acid diesters having 2 to 6 carbon atoms in the acidic component and 1 to 4 carbon atoms in the alcoholic component; (poly)ethylene glycols and ether derivatives and ester derivatives thereof, and poly(4-styrene sulfonic acid-co-maleic acid) sodium salt solution. The present invention further relates to a kit for carrying out the method of the invention.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.