Process for chromosomal integration and DNA sequence replacement in clostridia
US8628967B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Mar 20, 2012 |
| Grant date | Jan 14, 2014 |
| Priority date | — |
| Expiry date | Mar 20, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/74
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.