Protein/peptide sequencing by chemical degradation in the gas phase
US8628974B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 15, 2005 |
| Grant date | Jan 14, 2014 |
| Priority date | — |
| Expiry date | Apr 3, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6848
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fast and sensitive method and device for protein sequencing are disclosed. The method uses a combination of Edman degradation chemistry and mass spectrometry to sequence proteins and polypeptides. A peptide degradation reaction is performed on a polypeptide or protein ion reactant in the gas phase. The reaction yields a first ion product corresponding to a first amino acid residue of the polypeptide or protein reactant and a polypeptide or protein fragment ion. The mass-to-charge ratio for the first ion product, or the polypeptide or protein fragment ion, or both, is then determined. The first amino acid residue of the polypeptide or protein reactant is then identified from the mass-to-charge ratio so determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.