Nucleotide transient binding for sequencing methods

US8632975B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Peter Vander Horn · Foster City, US
• Cheng-Yao Chen · San Diego, US
• Guobin Luo · Oceanside, US
• Michael Previte · San Diego, US
• Jamshid Temirov · Germantown, US
• Theo Nikiforov · Carlsbad, US
• Zhaohui Zhou · San Ramon, US
• Hongye Sun · Karlsruhe, DE
• Yufang Wang · Nanhu, CN
• Stefanie Yukiko Nishimura · Mountain View, US
• Hongyi Wang · Pearland, US
• Marian Peris · San Mateo, US
• Barnett Rosenblum · San Jose, US
• Michael Phelan · Millbrae, US

Key dates

• Filing date: May 28, 2010
• Grant date: Jan 21, 2014
• Expiry date: Feb 4, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.