Assays

US8633013B2 · kind B2 · utility

Assignee

• CLONDIAG GMBH · DE

Inventors

• Thomas Kaiser · Hohlstedt, DE
• Klaus-Peter Mobius · Förderstedt, DE
• Torsten Schulz · Pinneberg, DE
• Thomas Uhlig · Dresden, DE
• Alexander Von Schenk Zu Schweinsberg · Jena, DE
• Eugen Ermantraut · Jena, DE
• Jens Tuchscheerer · Jena, DE

Key dates

• Filing date: May 5, 2008
• Grant date: Jan 21, 2014
• Expiry date: Mar 16, 2030

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0481
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte.