Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
US8637281B2 · kind B2 · utility
33Cited by
6References
21Claims
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Key dates
| Filing date | Sep 29, 2009 |
| Grant date | Jan 28, 2014 |
| Priority date | — |
| Expiry date | Sep 29, 2029 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0.1 μmol min−1 mg−1. LAB cells with even higher activities of 0.2 to 0.6 μmol min−1 mg−1 of DHAD activity were obtained.These modified cells may be used to produce isobutanol when additional isobutanol biosynthetic pathway enzymes are expressed.
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