Thermostable chimeric nucleic acid polymerases and uses thereof

US8637288B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Dirk Löffert · Mülheim an der Ruhr, DE
• Andreas Missel · Mülheim an der Ruhr, DE
• Jie Kang · Bonn, DE

Key dates

• Filing date: Aug 8, 2002
• Grant date: Jan 28, 2014
• Expiry date: Aug 8, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as DNA polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized. It is demonstrated that chimeric nucleic acid polymerases of the present invention possess the chemical and physical properties of their component domains (e.g., exonuclease activity, thermostability) and that the chimeric polymerases are thermostable.