Serial-line-scan-encoded multi-color fluorescence microscopy and imaging flow cytometry
US8639012B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 18, 2010 |
| Grant date | Jan 28, 2014 |
| Priority date | — |
| Expiry date | Apr 23, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6421
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system for performing high-speed, high-resolution imaging cytometry utilizes a line-scan sensor. A cell to be characterized is transported past a scan region. An optical system focuses an image of a portion of the scan region onto at least one linear light sensor, and repeated readings of light falling on the sensor are taken while a cell is transported though the scan region. The system may image cells directly, or may excite fluorescence in the cells and image the resulting light emitted from the cell by fluorescence. The system may provide a narrow band of illumination at the scan region. The system may include various filters and imaging optics that enable simultaneous multicolor fluorescence imaging cytometry. Multiple linear sensors may be provided, and images gathered by the individual sensors may be combined to construct an image having improved signal-to-noise characteristics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.