Use of thioacetic acid derivatives in the sulfurization of oligonucleotides with phenylacetyl disulfide
US8642755B2 · kind B2 · utility
58Cited by
8References
26Claims
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Key dates
| Filing date | Jun 30, 2009 |
| Grant date | Feb 4, 2014 |
| Priority date | — |
| Expiry date | Apr 30, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/02
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method and compositions for sulfurizing at least one phosphite or thiophosphite linkage in an oligonucleotide. The methods employ a phenylacetyl disulfide reagent (known as PADS), phenylthioacetic acid (PTAA) in the presence or absence or N-alkyl imidazole in industrially preferred solvents or solvents that are derived from renewable resources. The use of PTAA eliminates the need to “age” the PADS solution prior to its use in sulfurization reactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.