Methods for the identification of agents that inhibit mesenchymal-like tumor cells or their formation
US8642834B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 26, 2010 |
| Grant date | Feb 4, 2014 |
| Priority date | — |
| Expiry date | Feb 17, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5044
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides tumor cell preparations for use as models of the EMT process for use in the identification of anti-cancer agents, wherein said tumor cell preparations comprise cells of the epithelial tumor cell line CFPAC-1, which are stimulated by receptor ligands to induce EMT, or which have been engineered to inducibly express a protein that stimulates EMT. The present invention also provides methods of identifying potential anti-cancer agents by using such tumor cell preparations to identify agents that inhibit EMT, stimulate MET, or inhibit the growth of mesenchymal-like cells. Such agents should be particularly useful when used in conjunction with other anti-cancer drugs such as EGFR and IGF-1R kinase inhibitors, which appear to be less effective at inhibiting tumor cells that have undergone an EMT.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.