Patent · US Active

Artificial chromosome vector

US8647841B2 · kind B2 · utility

3Cited by
1References
19Claims
0Family size

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Key dates

Filing dateNov 18, 2009
Grant dateFeb 11, 2014
Priority date—
Expiry dateFeb 2, 2030

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N15/902
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

According to the present invention there is provided a method of producing a protein in a eukaryotic cell line, comprising the steps of a) providing a backbone of an artificial chromosome, b) recombining the nucleic acid encoding said protein into said backbone to generate an expression vector, c) introducing said expression vector into a eukaryotic host cell line to obtain a eukaryotic expression cell line, d) cultivating said expression cell line to produce said protein, and e) isolating said protein. The invention further relates to respective vectors and transgenic cell lines.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.