Patent · US Active

Minicircle vector production

US8647863B2 · kind B2 · utility

0Cited by

1References

22Claims

0Family size

Assignees

Gerhard Jechlinger · AT
Peter Mayrhofer · AT

Inventors

Peter Mayrhofer · Österreich, AT
Wolfgang Jechlinger · Wien, AT
Werner Lubitz · Klosterneuburg, AT

Key dates

Filing date	May 4, 2004
Grant date	Feb 11, 2014
Priority date	—
Expiry date	Jul 21, 2028

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/79
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A plasmid is provided comprising the following functional units: a prokaryotic origin of replication, a marker sequence, two specific recombinase recognition sequences and a multiple cloning site, whereby it comprises a gene coding for a sequence specific recombinase, whereby the units are arranged on the plasmid in such a way that the plasmid is divided into a miniplasmid and a minicircle upon expression of the sequence specific recombinase, said miniplasmid comprising the prokaryotic origin of replication, the marker sequence and the gene for the sequence specific recombinase and said minicircle comprising the multiple cloning site.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.